食品科学2017，Vol.38Issue(18)：8-16,9.DOI:10.7506/spkx1002-6630-201718002
居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化
Optimization of Bacterial Cellulose Production by Fermented Soybean Molasses with Komagataeibacter intermedius
摘要
Abstract
In this study, Komagataeibacter intermedius CGMCC12562 was employed to produce bacterial cellulose (BC) from soybean molasses. The fermentation process was optimized to improve BC yield. Through one-factor-at-a-time experiments, the soluble solids content (SSC) of soybean molasses, and corn gluten meal, ZnSO4 and malic acid were selected as the factors with a significant influence on BC production for optimization by response surface methodology. It was found that the optimized culture medium consisted of soybean molasses with an SSC level of 14.13 °Brix, 1.6% corn gluten meal, 0.11% ZnSO4 and 0.41% malic acid. The BC yield was as high as (15.68 ± 0.82) g/L after 7 days of culture at 30 ℃ for in a 250-mL conical flask containing 16% of the medium with an inoculum size of 10% at an initial pH of 6.0 under static cultivation conditions.关键词
大豆糖蜜/细菌纤维素/居间驹形氏杆菌/培养基/优化Key words
soybean molasses/bacterial cellulose/Komagataeibacter intermedius/medium/optimization分类
轻工纺织引用本文复制引用
尹园,马佳歌,倪春蕾,程建军,徐速..居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化[J].食品科学,2017,38(18):8-16,9.基金项目
"十二五"国家科技支撑计划项目(2014BAD22B01) （2014BAD22B01）
