食品科学2017，Vol.38Issue(18)：73-79,7.DOI:10.7506/spkx1002-6630-201718012
涡旋辅助液液微萃取结合GC-MS法检测67种白酒中四甲基吡嗪、4-甲基愈创木酚和4-乙基愈创木酚
Determination of Tetramethylpyrazine-4-Methyl Guaiacol and 4-Ethyl Guaiacol in 67 Chinese Baijiu Samples by Vortex Assisted Liquid-Liquid Microextration Combined with Gas Chromatography-Mass Spectrometry
摘要
Abstract
Tetramethylpyrazine, 4-methyl guaiacol and 4-ethyl guaiacol in 67 Chinese Baijiu samples were detected by vortex assisted liquid-liquid microextration (VALLME) combined with gas chromatography-mass spectrometry (GC-MS). The analytes were extracted with CH2Cl2, using a vortex agitator for 1 min, and then centrifuged at 6000 r/min for 5 min. After that, the CH2Cl2 phase was directly injected into GC-MS for analysis. The results indicated that the calibration curves for the three analytes displayed a good linearity (R2 > 0.99) in the ranges of 0.5–2500, 1.0–2500, and 1.0–2500 μg/L, respectively. The limits of detection (LODs) for tetramethylpyrazine, 4-methylguaiacol and 4-ethyl guaiacol were 0.25, 0.5 and 0.5 μg/L, respectively. The recoveries were in the range of 80.1%–88.0%. The method was successfully employed to detect the analytes in 67 Chinese Baijiu samples and the results showed that all the samples contained 1.5–2434.3 μg/L tetramethylpyrazine, of which 51 contained 2.8–1709.0 μg/L 4-methylguaiacol, and 53 contained 1.3–1167.5 μg/L 4-ethyl guaiacol. The differences in the contents of the health-beneficial ingredients in Chinese Baijiu may be related to the raw materials and manufacturing process.关键词
白酒/四甲基吡嗪/4-甲基愈创木酚/4-乙基愈创木酚/涡旋辅助液液微萃取(VALLME)法Key words
Baijiu/tetramethylpyrazine/4-methyl guaiacol/4-ethyl guaiacol/vortex assisted liquid-liquid microextration (VALLME)分类
轻工纺织引用本文复制引用
孙啸涛,王宗元,刘淼,敖灵,孙宝国,孙金沅,郑福平,黄明泉,李贺贺..涡旋辅助液液微萃取结合GC-MS法检测67种白酒中四甲基吡嗪、4-甲基愈创木酚和4-乙基愈创木酚[J].食品科学,2017,38(18):73-79,7.基金项目
国家自然科学基金青年科学基金项目(31601556) （31601556）
"十三五"国家重点研发计划重点专项(2016YFD0400501) （2016YFD0400501）
四川省科技计划项目(2017JZ0012) （2017JZ0012）
