食品科学2017，Vol.38Issue(18)：106-112,7.DOI:10.7506/spkx1002-6630-201718017
1种水溶性霸王花多糖的分离纯化及结构鉴定
Isolation, Purification and Structural Characterization of a Water-Soluble Polysaccharide from the Flower of Hylocereus undatus (Haw.) Britton & Rose
摘要
Abstract
Objective: To extract and purify polysaccharide fractions from the flower of Hylocereus undatus (Haw.) (H. undatus) Britton & Rose and investigate their physicochemical, spectroscopic, and structural properties. Methods:The crude water-soluble polysaccharide (HUP) was extracted, precipitated, deproteinized and lyophilized from flower of H. undatus. A homogeneous polysaccharide fraction, designated HUP0, was obtained by consecutive DEAE-cellulose and Sephadex G-100 column chromatography. Structural characterization of the polysaccharide fraction was conducted using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detection (HPSEC-MALLS-RID), Fourier transform infrared (FT-IR) spectroscopy, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy. Results: The weight-average molecular mass, intrinsic viscosity, specific rotation and mean diameter of HUP0 were measured to be 33.24 kD, 47.16 mL/g, +69°, and 196.96 nm, respectively. The FT-IR spectra showed that HUP0 was a neutral polysaccharide, which mainly contained β-configuration. Methylation, HPLC and NMR analyses indicated that HUP0 was a (1→4)-β-D-galactan.关键词
霸王花/多糖/结构特征/半乳聚糖Key words
flower of Hylocereus undatus (Haw.) Britton & Rose/polysaccharide/structural characterization/galactan分类
轻工纺织引用本文复制引用
何慕雪,孟凡成,王春明,李绍平,王英,叶文才,张庆文..1种水溶性霸王花多糖的分离纯化及结构鉴定[J].食品科学,2017,38(18):106-112,7.基金项目
科技部港澳台科技合作专项(2013DFM30080) （2013DFM30080）
澳门科学技术发展基金项目(042/2014/A1 （）
013/2008/A1) （）
