食品科学2017，Vol.38Issue(18)：187-192,6.DOI:10.7506/spkx1002-6630-201718030
凡纳对虾原肌球蛋白硫酸铵沉淀分离纯化方法的优化
Optimization of Purification by Ammonium Sulfate Precipitation of the Major Allergen Tropomyosin from Shrimp (Litopenaeus vannamei)
摘要
Abstract
Tropomyosin (TM), the major shrimp allergen, is one of the serious food safety problems throughout the world and has drawn much attention. It has been reported that the isolation and purification of TM is of very importance in the identification and control of allergy. In the present study, tropomyosin was purified from Litopenaeus vannamei by an improved method. The results indicated that TM could be purified effectively by adjusting the extraction buffer to pH 6.5 to 7.5 and then adding ammonium sulfate to approximately 30% saturation. The concentration of TM was about 43 μg/μL, as determined by a BCA Protein Assay Kit. The improved method avoided further purification of TM using high performance liquid chromatography (HPLC) or another chromatographic technology. In addition, the process of TM isolation was simplified and TM loss and dilution were avoided during further purification. High concentration of purified TM solution obtained in the present study could be directly used to study its physicochemical properties and allergenicity without being freeze-dried.关键词
过敏原/南美白对虾/原肌球蛋白/分离/纯化Key words
allergen/Litopenaeus vannamei/tropomyosin (TM)/isolation/purification分类
轻工纺织引用本文复制引用
傅玲琳,富舒洁,王彦波,王翀,张岩,吴琦,马爱进..凡纳对虾原肌球蛋白硫酸铵沉淀分离纯化方法的优化[J].食品科学,2017,38(18):187-192,6.基金项目
国家自然科学基金面上项目(31571770) （31571770）
"十三五"国家重点研发计划重点专项(2016YFD0401203) （2016YFD0401203）
浙江省科技厅公益技术应用研究计划项目(2016C32058) （2016C32058）
