食品科学2017，Vol.38Issue(18)：208-213,6.DOI:10.7506/spkx1002-6630-201718033
虾夷扇贝多糖提取及纯化方法的优化
Optimization of Extraction and Purification of Polysaccharides from Patinopecten yessoensis
摘要
Abstract
In this paper, the ultrasonic-assisted enzymatic extraction and purification of polysaccharides from Patinopecten yessoensis were optimized using one-factor and orthogonal array design methods. For the deproteinization of crude polysaccharides, D-glucono-δ-lactone (GDL) was used in this study for the first time and its performance was evaluated in comparison with trichloroacetic acid (TCA). The results showed that the optimum conditions for extraction were as follows:solvent/solid ratio, 40:1 (mL/g); enzyme dosage, 2.5%; and 3 h extraction at 60 ℃, resulting in the highest polysaccharide yield of 10.90%. The optimum conditions for TCA deproteinization were as follows: deproteinization time, 5 h; final TCA concentration, 5%; and 3 deproteinization cycles. The optimal conditions for GDL deproteinization were as follows:deproteinization time, 1 h; reaction temperature, 45 ℃; and final concentration, 0.3%. Three parallel experiments conducted under the optimized conditions gave an average deproteinization rate of 88.654% and 99.062% with relative standard derivation (RSD) of 0.905% and 0.679% for TCA and GDL, respectively. Comparison of the two deproteinization methods showed that the deproteinization efficiency of GDL method was higher and more stable.关键词
虾夷扇贝/多糖/三氯乙酸/D-葡萄糖酸-δ-内酯/脱蛋白Key words
Patinopecten yessoensis/polysaccharide/trichloroacetic acid/D-glucono-δ-lactone/deproteinization分类
轻工纺织引用本文复制引用
刘瑀,方志强,刘宇馨,娄亚迪..虾夷扇贝多糖提取及纯化方法的优化[J].食品科学,2017,38(18):208-213,6.基金项目
"十二五"国家科技支撑计划项目(2015BAD17B05) （2015BAD17B05）
