食品科学2017，Vol.38Issue(18)：214-221,8.DOI:10.7506/spkx1002-6630-201718034
超声波辅助纤维素酶提取石榴幼果多酚及其抑制α-葡萄糖苷酶活性
Optimization of Ultrasonic-Assisted Cellulase-Based Extraction and Anti-α-Glucosidase Activity of Polyphenols from Pomegranate Fruitlets
摘要
Abstract
This study aimed to optimize the ultrasonic-assisted cellulase-based extraction of polyphenols from pomegranate fruitlets. The factors with a significant influence on the yield of polyphenols were selected using a combination of one-factor-at-a-time method and Plackett-Burman (PB) design for the optimization experiments which were carried out using Box-Behnken design combined with response surface methodology. The anti-α-glucosidase activity and kinetics characteristics of the polyphenols were determined by using α-glucosidase inhibition model in vitro. Results showed that under the following conditions: ultrasonic power, 318 W; enzyme dosage, 23 U/mL; hydrolysis temperature, 46 ℃; and hydrolysis time, 2.7 h, the average yield of polyphenols from pomegranate fruitlets was 11.65%, close to the predicted value. The polyphenols had a strong anti-α-glucosidase activity. The percentage inhibition of α-glucosidase by the polyphenols at a concentration of 1.20 mg/mL reached 70.3% and the half inhibitory concentration (IC50) was 0.747 mg/mL. There was a positive relationship between the anti-α-glucosidase activity and polyphenol concentration in the range of 0.24–1.20 mg/mL. The inhibition was reversible and non-competitive.关键词
石榴幼果/多酚/超声波/纤维素酶/抑制α-葡萄糖苷酶活性Key words
pomegranate fruitlet/polyphenol/ultrasonic-assisted extraction/cellulose/anti-α-glucosidase activity分类
医药卫生引用本文复制引用
王占一,张立华,王玉海,戴博,郑丹丹,李卓瓦..超声波辅助纤维素酶提取石榴幼果多酚及其抑制α-葡萄糖苷酶活性[J].食品科学,2017,38(18):214-221,8.基金项目
2015年度国家星火计划项目(2015GA740027) （2015GA740027）
山东省自然科学基金项目(ZR2013BL018) （ZR2013BL018）
山东省重点研发计划项目(2016GSF202010) （2016GSF202010）
