食品工业科技Issue(13)：13-18,6.DOI:10.13386/j.issn1002-0306.2017.13.003
壳二糖、壳三糖单体制备及其结构分析
Monomer preparation and structure analysis of chitobiose and chitotriose
摘要
Abstract
Chitooligosaccharides has a variety of bioactivities due to its special structure features.Currently,almost all of chitooligosaccharides used in activity research were mixtures of polymers with different degrees of polymerization (DP).In this study,the specific chitosan enzyme was used to degrade chitosan to prepare chitobiose and chitotriose.The TLC analysis was then used to determine the end point of enzymatic hydrolysis reaction,and the component of hydrolysis products were analyzed by HPLC.The results showed that the enzymatic hydrolysates consisted of chitobiose(65.63％)and chitotriose(32.48％).Then,chitobiose and chitotriose were separated by a home-made cation ion exchange resin (QY-H003) with hydrochloric acid solutions at different concentrations(C1 =1.25 mol/L,C2 =1.55 mol/L).Further analysis was developed by TLC,HPLC,FT-IRand 1HNMR.The purity of separated chitobiose and chitotriose were 98.06％ and 96.00％,respectively.The recovery rate was 92.46％ and 95.53％,respectively.It indicates that the preparation process was suited for scale production of chitobiose and chitotriose with high purity,and it benefits for the future bioactivity studies and applications of chitooligosaccharides.关键词
壳聚糖/酶解/离子交换色谱法/壳二糖/壳三糖Key words
chitosan/enzymatic hydrolysis/ion exchange chromatography/chitobiose/chitotriose分类
轻工纺织引用本文复制引用
徐庆,陈列欢,秦臻,陈启明,邱勇隽,赵黎明..壳二糖、壳三糖单体制备及其结构分析[J].食品工业科技,2017,(13):13-18,6.基金项目
国家自然基金项目(31371725) （31371725）
上海市曙光计划(15SG28). （15SG28）
