食品工业科技Issue(13)：36-41,6.DOI:10.13386/j.issn1002-0306.2017.13.007
接骨草总黄酮提取工艺及其抑菌作用的研究
Study on the extraction and antibacterial effect of total flavonoids from Sambucus chinensis Lindl
摘要
Abstract
By using alkali soluble and acid sinking method,total flavonoids of Sambucus chinensis Lindl were extracted,and its antibacterial effect were studied in this paper.The extraction conditions including solid-liquid ratio,extraction temperature,extraction time,pH of xetraction liquid of flavonoids from Sambucus chinensis Lindl were optimized by single factor experiments and orthogonal experiments,and meanwhile the sinking efficiency of total flavonoids were studied by acidic pH precipitation,and the antibacterial activity of total flavonoids was studied by filter paper discs method and turbidimetric method.The results showed that the optimum extraction conditions of total flavonoids of Sambucus chinensis Lindl were as follow:solid-liquid ratio 1∶40 (g/mL),extraction temperature 80 ℃,extraction time 45 min,extraction liquid pH10,in which the extraction rate of total flavonoids of Sambucus chinensis Lindl was 3.11％.Adapting the extract to pH3.5 with HC1 could better precipitate about 2.15 ％ total flavonoids of Sambucus chinensis Lindl.Total flavonoids of Sambucus chinensis Lindl had a stronger antibacterial activity on Staphylococcus aureus,Bacillus subtilis,Escherichia coli at acidic pH4～5 than at neutral pH and alkaline pH value,and these bacteria conductivity was increased by total flavonoids.The minimum inhibitory concentration of total flavonoids of Sambucus chinensis Lindl on three bacteria were 0.63,1.25,2.50 mg/mL,respectively.The extracting process of total flavonoids from Sambucus chinensis Lindl by using alkali soluble and acid sinking method was simple and inexpensive,and its total flavonoids had a great antibacterial activity,in which played a foundation on the development and utilization of total flavonoids of Sambucus chinensis Lindl.关键词
接骨草/总黄酮/提取工艺/抑菌作用Key words
Sambucus chinensis Lindl/flavonoids/extraction process/antibacterial effect分类
轻工纺织引用本文复制引用
黄国文,管天球,赵雨云,陈莫林,刘宏辉..接骨草总黄酮提取工艺及其抑菌作用的研究[J].食品工业科技,2017,(13):36-41,6.基金项目
湖南省科技计划项目(2014NK2021) （2014NK2021）
湘南优势植物资源综合利用湖南省重点实验室资助项目(XNZW15C19). （XNZW15C19）
