食品工业科技Issue(13)：119-123,129,6.DOI:10.13386/j.issn1002-0306.2017.13.022
真菌Simplicillium lanosoniveum抗生素的分离纯化和初步表征
Isolation, purification and preliminary characterization of antibiotic from Simplicillium lanosoniveum
摘要
Abstract
The cyanobacterium-symbiotic fungus Simplicillium lanosoniveum was used as materials,and the objective of this study was to optimize the culture condition,purify and characterize a novel antibiotic which exhibitied antibacterial activity against the gram-positive bacteria from Simplicillium lanosoniveum.The suitable medium for this antibiotic production was the modified sabouraud medium containing sucrose 30 g/L and peptone 12 g/L and antibiotic production of Simplicillium lanosoniveum was non-growth-associated.This antibiotic displayed a characteristic absorption peak at 254 nm.The molecular weight of this antibiotic was 163.0182 with a retention time of 4.1031 min when the mobile phase of this antibiotic was a 0.02 mol/L carbonate buffer(pH7.5)containing 5％ acetonitrile.Biuret and ninhydrin tests of this antibiotic were negative.The founding of this antibiotic provide the feasibility for the classification of Simplicillium lanosoniveum.关键词
抗生素/Simplicillium lanosoniveum/纯化/表征Key words
antibiotic/Simplicillium lanosoniveum/purification/characterization分类
轻工纺织引用本文复制引用
董庆霖,董荣真,邢向英,苗佳旭,李玉宽..真菌Simplicillium lanosoniveum抗生素的分离纯化和初步表征[J].食品工业科技,2017,(13):119-123,129,6.基金项目
河北省自然科学基金项目(B2008000029). （B2008000029）
