食品工业科技Issue(13)：124-129,6.DOI:10.13386/j.issn1002-0306.2017.13.023
板栗过氧化氢酶基因CAT1的克隆及生物信息学分析
Cloning and bioinformatics analysis of catalase gene(CAT1)from Castanea mollissima blume
葛颖 1苗英杰 1吴祖芳 1翁佩芳 1张鑫1
作者信息
- 1. 宁波大学食品科学与工程系,应用海洋生物技术教育部重点实验室,浙江宁波315211
- 折叠
摘要
Abstract
The objectives of the present study were to provide a molecular mechanism basis in the studying of quality changes in postharvest and storage from Castanea mollissima blume,and further exploring the function of CAT1 gene.Full-length CAT1 cDNA was cloned from Castanea mollissima blume using rapid amplication of cDNA ends (RACE) method,and were analyzed by bioinformatics technology.The results of sequence analysis indicated that CAT1 had a length of 1485 bp containing a 1479 bp open reading frame which encoded 492 amino acid residues.Amino acid sequence analysis indicated that,CAT1 was high identity with the catalases of Ziziphus jujuba,Nicotiana tabacum,Gossypium hirsutum and many other plants.The predicted molecular weight of the protein was 56947.31 Da with the pI of 6.65.Characteristic analysis of protein results revealed that CAT1 protein is a stable,non-transmenbrane and hydrophilic protein,located in microbody (peroxisome).It has no signal peptide and contains 30 phosphorylation sites.The conserved domains service analysis indicated that CAT1 belonged to catalase-like superfamily.The secondary structure prediction showed that Castanea mollissima blume CAT1 protein mainly contains α-helix and random coil.Castanea mollissima blume CAT1 gene were cloned in this study,which laid the foundation for further studying biological of the gene.关键词
板栗/CAT1基因/克隆/生物信息学分析Key words
Castanea mollissima blume/catalase gene/cloning/bioinformatics分类
轻工纺织引用本文复制引用
葛颖,苗英杰,吴祖芳,翁佩芳,张鑫..板栗过氧化氢酶基因CAT1的克隆及生物信息学分析[J].食品工业科技,2017,(13):124-129,6.
