食品工业科技Issue(13)：186-190,5.DOI:10.13386/j.issn1002-0306.2017.13.035
大孔树脂纯化菠萝蜜果皮黄酮工艺
Purification of flavonoids from jackfruit peel with macroporous resin
摘要
Abstract
The best macroporous resin for purification jackfruit peel flavonoids was selected from five types resin by adsorption and desorption capacities.Based on single factor test,an orthogonal design was applied to optimize purification process.Analysis purification effect was evaluated by DPPH and ABTS radical scavenging assay.The results showed that NKA-9 macroporous resin was feasible for the purification.During the resin adsorption process,the optimum adsorption conditions were sample concentration 6 mg/mL,flow rate 1.5 mg/mL.The desorption conditions were elution concentration 70％ (v/v) alcohol,with eluanting velocity 2.5 mL/min.The optimized purification increased the purity of the flavonoid extract from jackfruit peel to 80.15％.The IC50 of the final purified product for scavenging DPPH and ABTS free radicals were 0.0054,0.015 mg/mL,much better than 0.041,0.092 mg/mL of crude flavonoids.The results suggested that NKA-9 type macroporous resin can be used to purify the flavonoids from jackfruit peel.关键词
大孔树脂/菠萝蜜果皮黄酮/纯化Key words
macroporous resin/flavonoids from jackfruit peel/purification分类
轻工纺织引用本文复制引用
段宙位,谢辉,何艾,王世萍,刘佳,窦志浩..大孔树脂纯化菠萝蜜果皮黄酮工艺[J].食品工业科技,2017,(13):186-190,5.基金项目
海南省省属科研院所技术开发研究专项(KYYS-2015-10). （KYYS-2015-10）
