食品工业科技Issue(13)：244-247,4.DOI:10.13386/j.issn1002-0306.2017.13.046
HPLC法测定不同采收期的云南甜柿叶中三种黄酮的含量
Determination of three flavonoids in the leaves of Yunnan Diospyros kaki in different harvest periods by HPLC
摘要
Abstract
A HPLC method was developed for determinating the contents of Kaempferol-3-O-β-D-glucopyranoside,Kaempferol-3-O-β-D-galactopyranoside,and Quercetin-3-O-β-D-glucopyranoside in the leaves of Yunnan Diospyros kaki in different harvest periods.The extraction conditions were 45％ ethyl alcohol,the ratio of liquid-solid of 20∶ 1,extraction time of 90 min for 4 times.The HPLC elution was carried out using methanol-0.4％ phosphate system at a flow rate of 1.0 mL/min,the injection volume was 10 μL,and the detector wavelength was set at 265 nm.The RSD values of the three flavonoids were all less than 1 c％ and the average recoveries were all above 99％.This method showed good separation effect and high stability,and could be used for determining the contents of the three flavonoids in the leaves of Yunnan Diospyros kaki in different harvest periods.The result showed that the accumulation of them in leaves of Yunnan Diospyros kaki exhibited regularity,and the contents were increasing along with fruit growing.关键词
甜柿/高效液相色谱法/山奈酚-3-O-β-D-葡萄糖苷/山奈酚-3-O-β-D-半乳糖苷/槲皮素-3-O-β-D-葡萄糖苷Key words
Diospyros kaki/HPLC/Kaempferol-3-O-β-D-glucopyranoside/Kaempferol-3-O-β-D-galactopyranoside/Quercetin-3-O-β-D-glucopyranoside分类
轻工纺织引用本文复制引用
梅为云,李娜,刘录,陈继坤,黄靳翔,高汪荣,周志宏..HPLC法测定不同采收期的云南甜柿叶中三种黄酮的含量[J].食品工业科技,2017,(13):244-247,4.基金项目
国家自然科学基金(81060338) （81060338）
云南省自然科学基金(2010CD072,2013FA040). （2010CD072,2013FA040）
