食品工业科技2017，Vol.38Issue(14)：1-5,10,6.DOI:10.13386/j.issn1002-0306.2017.14.001
HPLC法测定四种食用坚果种子中还原型谷胱甘肽(GSH)和蛋白巯基(P-SH)含量
Dertermination of reduced glutathione (GSH) and thiols in proteins (P-SH)in 4 kinds of nuts by high performance liquid chromatography (HPLC)
摘要
Abstract
The contents of reduced glutathione(GSH) and protein thiols in prunus duleis,cashew nut,pistachio nut,macadamia nut were detected by pre-column derivatization HPLC method using 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) as derivatizing agent with concomitant cell rupture sample pretreatments.The results showed that detected nuts contained relatively abundant GSH and thiols in P-SH.The amounts detected were GSH (1.2303 ± 0.01126) μmol/g,P-SH (3.5414 ± 0.0531) μmoL/g for pistachio nut,GSH (0.3069 ± 0.0185) μmol/g,P-SH (1.9001 ± 0.0194) μmol/g for prenus duleis,GSH (1.5708 ± 0.0216) μmol/g,P-SH (0.5905 ± 0.0238) μmol/g for macadamia nut,GSH (0.3373 ± 0.0047) μmol/g,P-SH (2.2458 ± 0.0225) μmol/g for cashew nut respectively.The recoveries of the methods were 99.37％ ～101.45％,and detection limits were 0.1386 μmol/g for GSH and 0.2184 μmol/g for protein thiols.The results provided a useful data for the application of these nuts for the healthcare.关键词
高效液相色谱法/坚果/谷胱甘肽/巯基化合物Key words
HPLC/nuts/glutathione/thiol compounds分类
轻工纺织引用本文复制引用
刘畅,应译娴,邱岳,崔胜云..HPLC法测定四种食用坚果种子中还原型谷胱甘肽(GSH)和蛋白巯基(P-SH)含量[J].食品工业科技,2017,38(14):1-5,10,6.基金项目
国家自然科学基金资助项目(21165021). （21165021）
