食品工业科技2017，Vol.38Issue(14)：82-86,5.DOI:10.13386/j.issn1002-0306.2017.14.016
苦荞中D-手性肌醇的纯化及其抗氧化活性研究
Study on purification and antioxidant activity of D-chiro-inositol from tartary buckwheat
摘要
Abstract
The D-chiro-inositol was obtained by ultrasonic extraction from tartary buckwheat.The total D-chiro-inositol content was measured by sodium periodate oxidation method.The D-chiro-inositol was purified by activated carbon column.The composition of D-chiro-inositol extractions were analyzed by high performance liquid chromatography (HPLC) method.Moreover,the reducing power of D-chiro-inositol extractions on DPPH,hydroxyl radicals and superoxide anion free radical in vitro was measured.Results showed that the content of crude D-chiro-inositol extractions in tartary buckwheat only attained 0.129％,however,the content of purified buckwheat D-chiro-inositol by actived carbon column could be achieved 27.26％.The antioxidant ability of D-chiro-inositol extractions had higher DPPH free radical,superoxide anion free radical and hydroxyl free radical scavenging effects as well as good reducing capacity.Besides,the antioxidant ability of purified product was better than crude extracting from tartary buckwheat.关键词
苦荞/D-手性肌醇/活性炭/高效液相色谱法/抗氧化Key words
tartar buckwheat/D-chiro-inositol/activated carbon/HPLC/antioxidant activity分类
轻工纺织引用本文复制引用
胡园园,易若琨,王仲明,聂旭元,骞宇,赵欣..苦荞中D-手性肌醇的纯化及其抗氧化活性研究[J].食品工业科技,2017,38(14):82-86,5.基金项目
重庆市教委科技项目(KJ1714357) （KJ1714357）
重庆高校创新团队建设计划资助项目(CXTDX201601040) （CXTDX201601040）
重庆市工程技术研究中心建设项目(cstc2015yfpt_gcjsyjzx0027) （cstc2015yfpt_gcjsyjzx0027）
