食品工业科技2017，Vol.38Issue(14)：116-120,5.DOI:10.13386/j.issn1002-0306.2017.14.023
超声对木瓜蛋白酶酶解产物分子量分布的影响
Effect of ultrasonic on molecular weight distribution of papain hydrolyzate
摘要
Abstract
The influence of ultrasonic on the molecular weight and distribution of papain hydrolyzate was discussed in this paper.The enzymolysis products of free and immobilized enzymes were determined by high performance liquid chromatography (HPLC)with bovine serum albumin as substrate.The results showed that there were significant differences in the molecular weight and distribution of the hydrolyzate peptide between free and immobilized enzymes under ultrasound and non-ultrasound conditions.The molecular weight and distribution of free papain and immobilized papain hydrolyzate under ultrasonic conditions were found to be mainly distributed in the range of 180～1000 Da and 1000～5000 Da.The proportion of hydrolyzed peptides in immobilized papain under the condition of ＜ 180 Da was relatively large.关键词
游离酶/固定化酶/超声/高效液相色谱法/肽分子量分布Key words
free enzyme/immobilized enzyme/ultrasonic/high performance liquid chromatography/peptide molecular weight distribution分类
轻工纺织引用本文复制引用
张杰,丁琳,白鸽,郑德娟,曹雁平..超声对木瓜蛋白酶酶解产物分子量分布的影响[J].食品工业科技,2017,38(14):116-120,5.基金项目
国家自然科学基金项目(31371722) （31371722）
