食品工业科技2017，Vol.38Issue(14)：131-136,6.DOI:10.13386/j.issn1002-0306.2017.14.026
中度嗜酸放线菌YY21液体发酵产纤溶酶条件的研究
Study on liquid fermentation conditions for fibrinolytic enzyme production by middle acidophilic Actinomycete sp.YY21
摘要
Abstract
In the current study,liquid fermentation medium and culture conditions were studied for fibrinolytic enzyme production from middle acidophilic Actinomycete sp.YY21 by single factor experiment.The results showed that the fermentation medium consisted of 1.7％ of millet,2％ of glucose,0.2％ of calcium carbonate,0.5％ of sodium chloride and 0.6％ of peptone.The optimized fermentation conditions were as follows:50 mL liquid medium in 250 mL flasks,inoculation volume 5 ％,rotate speed 160 r/min,culture time 4 d and culture temperature 22 ℃.Under the optimized conditions,the degradation zone area of Actinomycete sp.YY21 product by liquid fermentation reached 164.38 mm2 in the fibrin plate,increasing by 88％ than that of original conditions(87.58 mm2).Meanwhile,the fermentation time was decreased by 3 d.The production of fibrinolytic enzyme from middle acidophilic Actinomycete sp.YY21 was enhanced effectively by study of liquid fermentation medium and culture conditions.关键词
中度嗜酸放线菌YY21/纤溶酶/液体发酵/发酵条件Key words
middle acidophilic Actinomycete sp.YY21/fibrinolytic enzyme/liquid fermentation/fermentation conditions分类
轻工纺织引用本文复制引用
邓永平,刘晓兰,韩杨,艾瑞波..中度嗜酸放线菌YY21液体发酵产纤溶酶条件的研究[J].食品工业科技,2017,38(14):131-136,6.基金项目
国家自然科学基金项目(31301414). （31301414）
