食品工业科技2017，Vol.38Issue(14)：156-161,166,7.DOI:10.13386/j.issn1002-0306.2017.14.031
利用纤维素酶降解琼脂糖的条件优化
Optimization of enzymolysis conditions for degradation of agarose by cellulase
李庆旺 1熊瑶 2蔡坤淇 1MIAO Song 2张龙涛 1郑宝东2
作者信息
- 1. 福建农林大学食品科学学院,福建福州350002
- 2. 中爱国际合作食品物质学与结构设计研究中心,福建福州350002
- 折叠
摘要
Abstract
In this paper,the conditions of cellulose enzymatic degradation of agarose were studied with the yield of reducing sugar as the index.Following single factor experiment,the enzymatic hydrolysis conditions of agarose were optimized by four factors(cellulase content,enzymolysis temperature,pH and enzymolysis time)Box-Behnken experiment design and response surface methodology(RSM),and a regression model was established for predicting the enzymatic hydrolysis yield at different levels of cellulase content,enzymolysis temperature,pH and enzymatic hydrolysis time.In addition,the enzymatic hydrolysate products were preliminary identified.The optimum enzymolysis parameter,including,the substrate content was 0.2％,the cellulase content was 1909.3 U/mL,the enzymolysis temperature was 50.0 ℃,the pH was 4.9,and the enzymolysis time was 24.2 h.Under these conditions,the enzymolysis rate of agarose reached 29.58％ ± 0.26％.The results of thin layer chromatography(TLC)showed that the main products were agar oligosaccharides with polymerization degree of 2～6.关键词
琼脂糖/响应面法/酶解条件/薄层色谱Key words
agarose/response surface methodology/enzymatic hydrolysis conditions/thin layer chromatography (TLC)分类
轻工纺织引用本文复制引用
李庆旺,熊瑶,蔡坤淇,MIAO Song,张龙涛,郑宝东..利用纤维素酶降解琼脂糖的条件优化[J].食品工业科技,2017,38(14):156-161,166,7.
