食品工业科技2017，Vol.38Issue(14)：162-166,5.DOI:10.13386/j.issn1002-0306.2017.14.032
四种蛋白酶酶解对桦褐孔菌多糖的α-葡萄糖苷酶抑制活性影响
Effects of four kinds of proteases on α-glucosidase inhibitory activity of polysaccharide from Inonotus obliquus
摘要
Abstract
The relationship between α-glucosidase inhibitory activity and the composition bond type of binding protein of high temperature water-extracted polysaccharides(HIOP) that extracted from Inonotus obliquus was studied.HIOP was enzymatically digested with four different proteases,and the effect of protease hydrolysis on its molecular weight composition and oα-glucosidase inhibitory activity was determined.The inhibitory rate of α-glucosidase by original HIOP was 83.72％ at 10 μg/mL,while the lowest α-glucosidase inhibition rate of HIOP that hydrolyzed by neutral protease,alkaline protease,pepsin,and trypsin were 53％,65％,6.5％ and 7.1％,respectively.The binding protein in HIOP had a role in the α-glucosidase inhibitory activity of HIOP.In addition,in consideration of the effects of these four kinds of enzymes,it could be speculated that the active sites of the binding proteins in HIOP contain aromatic amino acids,acidic amino acids,lysine or arginine.If we destroy such peptide bonds,the α-glucosidase inhibitory activity of HIOP could be significantly reduced.But,the four proteases did not change the molecular weight of HIOP,indicating that the four proteases only affected the binding site of HIOP to α-glucosidase activity center.关键词
桦褐孔菌粗多糖/蛋白酶/α-葡萄糖苷酶抑制活性Key words
the polysaccharide of Inonotus obliquus/proteases/α-glucosidase activity分类
轻工纺织引用本文复制引用
佟世生,胡锦荣,张京声,董文霞,王丽,刘萍..四种蛋白酶酶解对桦褐孔菌多糖的α-葡萄糖苷酶抑制活性影响[J].食品工业科技,2017,38(14):162-166,5.基金项目
国家自然基金项目(21576282) （21576282）
