食品工业科技2017，Vol.38Issue(14)：280-284,5.DOI:10.13386/j.issn1002-0306.2017.14.055
硒蛋白Se-76P对STZ诱导的小鼠糖尿病肾病模型肾功能及生化指标的影响
Effect of selenium Se-76P on STZ-induced renal function and biochemical indexes in mice with diabetic nephropathy
摘要
Abstract
To explore the role and mechanism of selenium protein (Se-76P)on streptozocin-induced diabeticnephropathy in mice.Diabetic nephropathy model were prepared with streptozocin (40 mg/kg)by intraperitoneal injection in male C57BL/6J male nmice.60 mice were divided into six groups,normal control group(group Ⅰ),model of diabetic nephropathy group(group Ⅱ),model + low-dose Se-76P group(group Ⅲ),model + middle-dose Se-76P group(group ⅣV),model + high dose Se-76P group(group V)and insulin treatment group (group VⅥ).Both groups of mice were administrated by subcutaneous injection way,group Ⅰ and group Ⅱ was given saline,group Ⅲ,ⅣV and V were given low (20 μ mol/kg),middle (40 μmol/kg) and high(60 μmol/kg)concentration of Se-76P protein,group VⅣ was given insulin,5 U/kg· d.All groups were treated for 4 weeks,and body weights were measured every week.After the experiment,blood glucose,serum creatinine (SCr) and urea nitrogen(BUN) levels were measured in serum.The urine creatinine(UCr) and microalbuminuria(MAU)levels were detected and the creatinine clearance (CCr) was calculated,renal tissue malondialdehyde (MDA),superoxide dismutase (SOD) and glutathione peroxidase(GPx) levels were also detected.Compared with model group,fasting blood sugar were decreased in Se76P administrated groups,the levels of SCr,BUN,UCr,MAU and CCr were significantly decreased(p ＜0.01 or 0.05).The MDA level was decreased in kidney tissues significantly(p ＜ 0.01),SOD and GPx activity were increased significantly (p ＜ 0.01 or p ＜0.05).Se-76P may improve renal function in STZ-induced diabetic nephropathy mice by increasing SOD and GPx activities,enhancing antioxidant levels.关键词
硒蛋白/Se-76P/链脲佐菌素/糖尿病肾病Key words
selenoprotein/Se-76P/streptozocin/diabetic kidney disease分类
轻工纺织引用本文复制引用
李强,田福俊,魏子敬,葛鑫,胡超,王程..硒蛋白Se-76P对STZ诱导的小鼠糖尿病肾病模型肾功能及生化指标的影响[J].食品工业科技,2017,38(14):280-284,5.基金项目
吉林省科技厅自然科学基金项目(20150101131JC) （20150101131JC）
吉林省教育厅“十二五”科学技术研究项目(吉教合字2015-405) （吉教合字2015-405）
吉林省卫生厅科技计划基金资助项目(2013ZC015). （2013ZC015）
