食品工业科技2017，Vol.38Issue(14)：294-298,303,6.DOI:10.13386/j.issn1002-0306.2017.14.058
桑葚粗提液对壬基酚诱导大鼠焦虑行为的干预作用及其机制
Intervention effects and mechanism research of mulberry crude extract on nonylphenol anxiety behavior of SD rats induced by nonylphenol
摘要
Abstract
The aim of this study was toinvestigate the interventioneffects of mulberry crude extract (MCE)on anxiety behaviors of SD rats exposed to nonylphenol(NP).48 SPF rats were randomly divided into 6 goups:normal control group (corn oil),MCE control group(120 mg/kg),NP model group(270 mg/kg),and three intervention groups(120 mg/kg NP + 30 mg/kg MCE,120 mg/kg NP + 60 mg/kg MCE,120 mg/kg NP + 120 mg/kg MCE).The open field test(OFT) and elevated plus maze(EPM)were used to evaluate anxiety behaviors of the rats while the ELISA kitswere used to detect the levels of monoamine neurotransmitter,including serotonin,dopamine,norepinephrine and monoamine oxidase.The distance traveled in the center and entrance frequency to the center of the high-dose intervention groupwere significantly higher thanthe NP group in the OFT.The percentage of open arm entries and time of the high-dose intervention group rats were significantly higher thanthe NP group in the EPM test(p ＜ 0.05).Compared with the nonylphenol group,the hypothalamic serotoninand dopamin levels in the high-dose intervention group were significantly lower(p ＜ 0.05),the hypothalamus dopamin level was significantly lower in the middledose intervention group(p ＜0.05)and MAO activity of high dose intervention group was significantly increased (p ＜ 0.05).Meanwhile as intervention dose increased,norepinephrine levels gradually reduce with no significant difference.Mulberry crude extract can reduce the levels of serotonin,dopamine,norepinephrine in hypothalamus of rats with anxiety caused by nonylphenol and the underlying mechanism may involve up-regulation of MAO activity.关键词
壬基酚/桑葚提取液/单胺类神经递质/旷场实验/高架十字迷宫Key words
nonylphenol/mulberry crude extract/monoamine neurotransmitter/open field text/elevated plus maze分类
轻工纺织引用本文复制引用
刘焕,杨婕,黄少文,刘瑞菁,贺永健,柳春红..桑葚粗提液对壬基酚诱导大鼠焦虑行为的干预作用及其机制[J].食品工业科技,2017,38(14):294-298,303,6.基金项目
广东省自然科学基金项目(2016A030313395) （2016A030313395）
农业部农产品质量安全监管项目(GJFP201601106) （GJFP201601106）
广东省科技计划项目(2013B020204001) （2013B020204001）
广东省现代农业产业技术体系创新团队建设项目(2016LM2152). （2016LM2152）
