食品与机械2017，Vol.33Issue(7)：26-30,5.DOI:10.13652/j.issn.1003-5788.2017.07.006
传统泡菜中抗性乳酸菌的筛选及鉴定
Screening and identification of resistant lactobacillus in traditional pickles
摘要
Abstract
40 strains of actic acid bacteria isolated by traditional pickled cabbage were regarded as the object.Through the tests of acid resistance,anti gastric juice and bile salt resistance,actic acid bacteria with good resistance in traditional pickles were determined,using 16S rDNA sequence and phylogenetic tree to analyze the genus relationship of good resistance of lactic acid bacteria.The experimental results showed that MFR-28 and MFR-30 facing to artificial gastric juice and bile salt had a strong tolerance in 40 strains of actic acid bacteria,and survival rates in the artificial gastric juice were 85.21 ％ and 90.47％ respectively,and the growth of efficiency in 0.3％ bile salt were 11.84％ and 7.37％ respectively;through the analysis of 16S rDNA sequence and phylogenetic tree,MFR-28 and MFR-30 were Lactobacillus casei.In this study,2 strains of resistant lactobacillus casei can be used as potential probiotics to further explore their physiological functions and to develop probiotic products.关键词
泡菜/乳酸菌/耐受能力/人工胃液/胆盐Key words
pickles/lactobacillus/tolerance/artificial gastric juice/bile salt引用本文复制引用
刘思雨,肖菁,索化夷..传统泡菜中抗性乳酸菌的筛选及鉴定[J].食品与机械,2017,33(7):26-30,5.基金项目
重庆市社会民生科技创新专项(编号:cstc2015shmszx80021) （编号:cstc2015shmszx80021）
重庆市特色食品工程技术研究中心能力提升项目(编号:cstc2014ptgc8001) （编号:cstc2014ptgc8001）
中央高校基本业务费专项资金(编号:XDJK2016A018) （编号:XDJK2016A018）
