食品与机械2017，Vol.33Issue(7)：56-60,5.DOI:10.13652/j.issn.1003-5788.2017.07.012
基于纳米金与适配体的赭曲霉毒素A检测方法研究
Study on detection method for ochratoxin A based on gold nanoparticle and aptamer
摘要
Abstract
A high sensitivity fluorescence detection method for ochratoxin A (OTA) based on OTA aptamers and gold nanoparticle (AuNPs) was established,which employed OTA aptamers as recognition element and AuNPs to quench the fluorescence emission from fluorescein.The results indicated that the detection limit is 10 nmol/L and a linear relationship between the fluorescent intensity ratio and the logarithm of OTA concentration was plotted in the range of 25 ～1 000 nmol/L (R2=0.991).The detection method showed high fea sibility and reliability.Compared with traditional method,this method greatly improved the detection efficiency and provided techni cal support for more effective identification of OTA.关键词
纳米金/适配体/赭曲霉毒素A/生物传感器Key words
gold nanoparticle/aptamer/ochratoxin A/biosensor引用本文复制引用
吕蕾,赵阳阳,刘仁杰,金永东,郭志军..基于纳米金与适配体的赭曲霉毒素A检测方法研究[J].食品与机械,2017,33(7):56-60,5.基金项目
吉林省科技厅青年科研基金项目(编号:20160520047JH) （编号:20160520047JH）
吉林省教育厅科学技术研究项目(编号:吉教科合字[2016]第252号) （编号:吉教科合字[2016]第252号）
国家自然科学基金项目(编号:31360384,31460423) （编号:31360384,31460423）
