食品与机械2017，Vol.33Issue(7)：65-69,154,6.DOI:10.13652/j.issn.1003-5788.2017.07.014
超声辅助提取-HPLC-ICP-MS同时测定水产及其制品中3种形态汞的含量
Simultaneous determination of the content of the different mercury speciation in aquatic products by liquid chromatography-inductively coupled plasma mass spectrometry coupled with ultrasonic-assisted extraction
摘要
Abstract
Ultrasonic assisted extraction coupled with high performance liquid chromatography-inductively coupled plasma mass spectrometry was developed for the determination of mercury speciation in aquatic products.Using ultrasonic assisted extraction for sample in HCl + L-cysteine system,with 10 mmol/L ammonium acetate +0.10％ L cysteine buffer and methanol (98 ∶ 2) system consisting of mobile phase,and detected the content of inorganic mercury and methyl mercury and ethyl mercury by high performance liquid chromatography inductively coupled plasma mass spectrometry.The detection limits (3S/N) of inorganic mercury,methyl mercury and ethyl mercury were 0.18,0.09,0.15 ng/mL,respectively.The correlation coefficient (R) of the regression equation of the standard curve was above 0.999.Inorganic mercury and methyl mercury,ethyl mercury were spiked in actual samples,the rate of recovery was 78.5 ％ ～ 96.8 ％,the relative standard deviation (RSD)≤ 3.0％.The standard substance was detected by this method,and the measured value was consistent with the standard value.The method is accurate,reliable,simple,rapid and accurate.It can be applied to the analysis of mercury speciation in aquatic products.关键词
无机汞/甲基汞/乙基汞/水产品/超声辅助提取/高效液相色谱/电感耦合等离子体质谱Key words
Inorganic mercury/methyl mercury/ethyl mercury/aquatic products ultrasonic-assisted extraction/HPLC/ICP-MS引用本文复制引用
乔晴,何兵兵,王向军,李辰,刘军红..超声辅助提取-HPLC-ICP-MS同时测定水产及其制品中3种形态汞的含量[J].食品与机械,2017,33(7):65-69,154,6.基金项目
河南省科技厅科技攻关项目(编号:162102310065) （编号:162102310065）
