食品与机械2017，Vol.33Issue(7)：149-154,6.DOI:10.13652/j.issn.1003-5788.2017.07.033
发芽糙米多糖双水相萃取工艺优化及其抗氧化活性
Extraction of polysaccharide from germinated brown rice by aqueous two-phase system and research of its antioxidant activity
摘要
Abstract
The polysaccharide was extracted by the ultrasonic assisted aqueous two-phase technology from germinated brown rice.The phase separation ability of different in PEG/(NH4)2 SO4 system was studied according to the polysaccharide yield.The yield of polysaccharide substance of germinated brown rice was determined by orthogonal experiment.The optimum process conditions were determined.Results were showed as follows:the mass fraction of PEG6000 was 15.7％,the mass fraction of ammonium sulfate was 14.8％ and extraction time was 40 min.The yield of polysaccharide substance from germinated brown rice was 81.07％ in the optimum conditions.Morever,the anti-oxidation of polysaccharide substance from germinated brown rice were studied by methods of DPPH,pyrogallol autoxidation and phenanthroline.The results showed that the polysaccharide of brown rice had stronger antioxidant effect to DPPH,ultra oxygen anion and hydroxyl radicals.关键词
发芽糙米/多糖/超声波/双水相萃取/抗氧化性Key words
Germinated brown rice/polysaccharide/ultrasonic/two-phase extraction/antioxidant activity引用本文复制引用
刘晓飞,王鑫,孟庆虹,张宇,龚丽丽,马永强,卢淑雯..发芽糙米多糖双水相萃取工艺优化及其抗氧化活性[J].食品与机械,2017,33(7):149-154,6.基金项目
黑龙江省博士后资助经费(编号:LBH-Z15200) （编号:LBH-Z15200）
黑龙江省教育厅科学技术研究项目(编号:12541189) （编号:12541189）
