茶叶科学2017，Vol.37Issue(5)：493-502,10.
茶树磷转运蛋白基因CsPT4的克隆、亚细胞定位及表达分析
Molecular Cloning, Subcellular Localization and Expression Analysis of CsPT4 Gene in Tea Plant (Camellia sinensis)
摘要
Abstract
Phosphate transporter proteins (Phts) play important roles in plant phosphorus (P) absorption and transportation. Furthermore, Phts affect usage efficiency of the tea garden fertilizer. A full-length phosphate transporter complementary DNA (cDNA) CsPht1:4 (also named CsPT4) was cloned from tea plant (Camellia sinensis cv. Longjingchangye) by rapid amplification of cDNA ends (RACE) techniques. CsPT4 had an open reading frame of 1620 bp (GenBank accession No. KY132100) and encoded a 539 amino acid polypeptide. Bioinformatic analyses showed that CsPT4 had a molecular weight of 59.12 kD and a theoretical isoelectric point of 8.51. The protein secondary structure was a "6+Hydrophilic+6" configuration,which was consistent with the typical structure of Phts. Subcellular localization assay showed that the CsPT4 protein localized in plasma membrane, which was consistent with the predicted results of Softberry. The expression pattern of CsPT4 gene was tissue-specific. Its transcript abundance in old leaves was much higher than that in tender leaves, stems and roots. The lowest expression of CsPT4 gene was identified in roots. Quantitative real-time PCR showed that the gene expression trends in root and leaves were different under low-P and P-deficiency treatments. Under low-P treatment, its induced level was first increased and then decreased, with higher expression in roots than leaves. While under P-deficiency treatment, the induced expression of CsPT4 gene kept stable, with its peak in roots and leaves at 72 h and 48 h, respectively. The results of this study provided a reference for the study of the molecular mechanism of tea adaptation to low P.关键词
茶树/磷转运蛋白/基因克隆/亚细胞定位/表达分析Key words
tea plant (Camellia sinensis)/phosphate transporter protein/gene cloning/subcellular localization/expression analysis分类
农业科技引用本文复制引用
辛华洪,王伟东,王明乐,马青平,甘玉迪,黎星辉..茶树磷转运蛋白基因CsPT4的克隆、亚细胞定位及表达分析[J].茶叶科学,2017,37(5):493-502,10.基金项目
现代农业产业技术体系建设专项资金(CARS-23)、江苏高校优势学科建设工程资助项目、国家自然科学基金(31570691)、江苏省科技支撑计划(BE2009313-1) （CARS-23）
