现代食品科技2017，Vol.33Issue(9)：20-27,8.DOI:10.13982/j.mfst.1673-9078.2017.9.004
集胞藻PCC6803中S2P蛋白酶Slr1821参与热胁迫响应的研究
Exploration of the Involvement of the S2P Protease Slr1821 of Synechocystis sp.PCC6803 in Heat Stress Response
摘要
Abstract
With the rising emission of carbon dioxide(CO2),the greenhouse effect is becoming increasingly obvious,and global warming has caused more severe damage to the growth of crops and the ecological environment.Thus,investigation of the response mechanism of photosynthetic autotmphs to heat stress is of scientific significance and has potential applications.Synechocystis sp.PCC6803 is a model cyanobacterium used to study photosynthesis and the response to abiotic stress.In this paper,the coding gene of the site-2-protease Slr1821 of Synechocystis sp.PCC6803 was knocked out completely by homologous recombination to generate the ⊿slr1821 mutant.The results showed that after heat treatment at 44 ℃,the ⊿slr1821 mutant could not grow at all,whereas the wild-type recovered from thermal damage quickly,indicating that Slr1821 protein played an important role in heat acclimation.Through analysis of changes in the relative content of phycobiliprotein and the content of chlorophyll,the results showed that heat treatment-induced deficiency ofslrl821 inhibited the resynthesis of photosynthetic pigments,including phycobiliprotein and chlorophyll.These findings provide important insights into analysis of the mechanisms through which photosynthetic autotrophs adapt to heat stress.关键词
温室效应/热胁迫/集胞藻PCC6803/slr1821Key words
greenhouse effect/heat stress/Synechocystis sp.PCC6803/slr1821引用本文复制引用
陈谷,李仕良,刘小芳,林诗琪,丁清龙..集胞藻PCC6803中S2P蛋白酶Slr1821参与热胁迫响应的研究[J].现代食品科技,2017,33(9):20-27,8.基金项目
国家自然科学基金委员会面上项目(31270085) （31270085）
