现代食品科技2017，Vol.33Issue(9)：56-62,7.DOI:10.13982/j.mfst.1673-9078.2017.9.008
柳蘑多糖对肿瘤细胞增殖抑制作用的研究
Inhibitory Effect of Polysaccharides from Pholiota adiposa on Tumor Cell Proliferation
摘要
Abstract
To study the effects of extraction solvent acidity on the preparation of Pholiota adiposa polysaccharides (e.g.,yield and purity) and their inhibitory effects on tumor cell proliferation,three types ofP.adiposa polysaccharides (SPAP1,WPAP1,and JPAP1) were prepared using 0.1 mol/L HC1 (aq),distilled water,and 0.1 mol/L sodium hydroxide (aq),respectively.The deproteinized P.adiposa polysaccharides (SPAP2,WPAP2,and JPAP2) were obtained from the deproteinization of SPAP1,WPAP1,and JPAP1,respectively.The effects of the extraction yield and purity of P.adiposa polysaccharides on the proliferation of tumor cells were investigated,and the differences in the structures of P adiposa polysaccharides were preliminarily analyzed by infrared spectrometry.The result indicated that the acidity of the extraction solvent considerably impacted the extraction yield and purity ofP adiposa polysaccharides,and the highest extraction yield and the highest purity were found in JPAP1 (11.02％) and SPAP2 (73.91％),respectively The inhibitory effect of SPAP2 on tumor cell proliferation was significantly higher than those of other P adiposa polysaccharides (p＜0.05) and dose-and time-dependent effects of polysaccharide treatment were observed.SPAP2 exhibited thestrongest inhibitory effect on the proliferation of HeLa cells,with an inhibition rate of 90.88％.Infrared spectroscopy results showed that SPAP2 had strong absorption peaks at 1078±4 cm~ and 759.83 cm-1,which indicated that the content of C-O-H and C-O-C groups in SPAP2 were higher than those in other polysaccharides.These results may provide a theoretical basis for further development ofP adiposa polysaccharide products and an in-depth study on the mechanism ofP adiposa polysaccharide-induced tumor cell apoptosis.关键词
柳蘑/多糖/提取溶剂酸碱性/肿瘤细胞Key words
Pholiota adiposa/polysaccharide/the type of solvent(PH)/tumor cell引用本文复制引用
周聪,孙月,李德海,顾嘉林,龚金华..柳蘑多糖对肿瘤细胞增殖抑制作用的研究[J].现代食品科技,2017,33(9):56-62,7.基金项目
中央高校基本科研业务费专项资金资助(2572014CA19) （2572014CA19）
黑龙江省自然科学基金面上项目(C2015062) （C2015062）
黑龙江省教育厅科学技术研究项目(12543015) （12543015）
