现代食品科技2017，Vol.33Issue(9)：63-70,19,9.DOI:10.13982/j.mfst.1673-9078.2017.9.009
高粱糠不同存在形态酚类物质的AB-8大孔树脂纯化及对红细胞氧化溶血的保护作用
Purification of Different Phenolic Compounds from Sorghum Bran on AB-8 Macroporous Resin and Protective Effects against Oxidative-stress-induced Erythrocyte Hemolysis
摘要
Abstract
Different types of phenols extracted from sorghum bran were purified on the AB-8 macroporous resin and then analyzed by high performance liquid chromatography (HPLC).The inhibitory effects of the phenols on human-erythrocyte hemolysis caused by 2,20-azobis-(2-amidinopropane) dihydrochloride (AAPH) were studied.The results showed that after purification on the AB-8 macroporous resin,phenol concentrations were in the following order:free phenols ＞ insoluble bound esterified phenols ＞ insoluble bound glycosidic phenols ＞ soluble glycosidic phenols ＞ soluble esterified phenols.According to the results of HPLC,the concentrations of 18 phenolic compounds were in the following order:insoluble bound esterified phenols ＞ free phenols ＞ insoluble bound glycosidic phenols ＞ soluble esterified phenols ＞ soluble glycosidic phenols.After the treatment with 200 μg/mL insoluble bound esterified,free,insoluble bound glycosidic,or soluble esterified phenols,the rate of AAPH-induced hemolysis of erythrocytes decreased to ～10％,which was close to that of the negative control group;the AAPH hemolysis rate of erythrocytes in the soluble glycosidic phenolic (200 μg/mL) treatrnent group was still 28.84％.Pretreatments with five different types of phenols effectively reduced the concentration of malondialdehyde (MDA),increased the glutathione/glutathione disulfide (GSH/GSSG) ratio in the AAPH-treated erythrocytes,and restored the erythrocytes to the normal state.The erythrocytes with relatively low superoxide dismutase (SOD),catalase (CAT),and glutathione peroxidase (GSH-Px) activities were also protected.In conclusion,the different types of phenols from sorghum bran after purification on the AB-8 macroporous resin may protect human erythrocytes from the damage caused by AAPH-generated free radicals,and their intracellular antioxidant activity is related to their total phenolic content and composition of a mixture of phenolic compounds.关键词
高粱糠/酚类物质/AB-8大孔树脂/抗氧化活性/人血红细胞Key words
sorghum bran/phenols/AB-8 macroporous resin/antioxidant activity/human erythrocytes引用本文复制引用
陈华敏,赖富饶,孙崇臻,张猛猛,吴晖,唐语谦,李晓凤..高粱糠不同存在形态酚类物质的AB-8大孔树脂纯化及对红细胞氧化溶血的保护作用[J].现代食品科技,2017,33(9):63-70,19,9.基金项目
中央高校基本科研业务费专项资助项目(2013ZM0065) （2013ZM0065）
广东省天然产物绿色加工与产品安全重点实验室开放基金资助项目(201304) （201304）
广东省高等学校科技创新重点项目(2012CXZD0009) （2012CXZD0009）
国家自然科学基金项目(31201330) （31201330）
广州市科技攻关项目(201300000202) （201300000202）
