食品工业科技2017，Vol.38Issue(15)：25-29,5.DOI:10.13386/j.issn1002-0306.2017.15.006
双效型酵母细胞壁提取物及其对黄曲霉毒素B1吸附特性研究
Double function yeast cell-wall extract and its capability of adsorbing aflatoxin B1
摘要
Abstract
In this study,we managed to produce a type of cell-wall extract with double functions i.e.spore inhibition and toxins adsorption by using thymol to anchor the phospholipid of yeast cells and lvse them.lt was showed that thymol led to the yeast cell death with the observation by using the flow cytometry and fluorescence microscope.When yeast cell-wall extract by yeast cell was treated with 500 μg/mL thymol,the content of β-glucan was increased by 51.5％ in the cell-wall extracts by phenol -sulfuric,and soluble protein in cells was decreased by 40％ by SDS-PAGE.This indicated that thymol gave rise to the lysis of yeast cell and destroyed the integrity of the cell membrane,the structural of peptidoglycan on the cell wall changed,yeast cell released their contentand and the adsorption capacity of AFB1 increased.The capability of cell-wall extract adsorbing AFB1 was 2.5 μg/mg by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC).And its thymolloading capacity was 41.5 μg/mg.This study provided new ideas and theoretical basis for the control of aflatoxin contamination in food and feed.关键词
麝香草酚/酵母细胞壁提取物/吸附/黄曲霉毒素B1Key words
thymol/yeast cell-wall extract/adsorption/aflatoxin B1分类
轻工纺织引用本文复制引用
钱潘攀,李红波,赵岩岩,周威,莫海珍,胡梁斌..双效型酵母细胞壁提取物及其对黄曲霉毒素B1吸附特性研究[J].食品工业科技,2017,38(15):25-29,5.基金项目
国家自然科学基金项目(31671952) （31671952）
河南省科技攻关计划项目(152102110086,162102110065) （152102110086,162102110065）
