食品工业科技2017，Vol.38Issue(15)：169-174,243,7.DOI:10.13386/j.issn1002-0306.2017.13.032
萃取-吸附法分离萃取发酵液中红曲黄色素
Extraction and adsorption of Monascus yellow pigments from extractive fermentation broth
摘要
Abstract
Extractive fermentation was an advanced technology for a high-yield productivity of Monascus pigments.Intracellular pigments could be extracted to the extracellular broth with surfactant Triton X-100.However,the solid bonding between pigments and Triton X-100 micelles challenged a subsequent separation and purification of the pigments from fermentation broth.This study demonstrated a selective isolation of Monascus yellow pigments from the extractive fermentation broth through a dual-phase chloroform extraction and subsequent adsorption using S-8 alkali resin.Recovery of the pigments and efficiency of Triton X-100 removal reached 64.5％ and 97.47％,respectively.The pigment adsorption could be described by Freundlich equilibrium model with a pseudo-second order kinetic course.The use of methanol solution containing 0.5 ％ (w/v) HC1 led to the highest elution efficiency with a total pigment recovery yield of 79.48％.The method was demonstrated to be a new approach for pigment isolation in extractive fermentation.关键词
红曲黄色素/大孔树脂/萃取分离/吸附分离/动力学Key words
Monascus yellow pigments/marcoporous resin/extraction/adsorption/kinetics分类
轻工纺织引用本文复制引用
唐锐,谭海玲,石侃,田霄飞,吴振强..萃取-吸附法分离萃取发酵液中红曲黄色素[J].食品工业科技,2017,38(15):169-174,243,7.基金项目
国家自然科学基金(31271925) （31271925）
广东省教育部产学研结合项目(2013B090600015) （2013B090600015）
广州市科技计划项目(2014J4100192). （2014J4100192）
