食品工业科技2017，Vol.38Issue(15)：212-217,6.DOI:10.13386/j.issn1002-0306.2017.15.040
二甲亚砜法破壁条件对法夫酵母虾青素提取效果的影响
Effects of disrupting conditions on extracting astaxanthin from Phaffia rhodozyma by DMSO method
摘要
Abstract
In order to evaluate the possibility of extracting astaxanthin from Phaffia rhodozyma disrupted cell wall by DMSO method,influences of several factors such as type of solvent,dosage of DMSO,temperature,the ratio of solid to liquid,extraction time and extracting times on recovery of astaxanthin from Phaffia rhodozyma were studied and conditions for disrupting the yeast cell wall were optimized.Results showed that acetone was the optimal solvent for extracting astaxanthin.The effect significance of extraction temperature,the ratio of solid to liquid and extraction time were gradually decreased on recovery of astaxanthin.The optimal conditions obtained from single factor and orthogonal experiment were as follows:acetone as the extracting solvent,1∶1.5 dosage of DMSO,2 times of extraction,45 ℃ of extraction temperature,1∶20 of the ratio of solid to liquid and 40 min of extraction time.Under the optimal conditions,the astaxanthin recovery from Phaffia rhodozyma was 4.42 mg/g.关键词
法夫酵母/虾青素/二甲亚砜/正交实验/提取Key words
Phaffia rhodozyma/astaxanthin/DMSO/orthogonal experiment/extraction分类
轻工纺织引用本文复制引用
黄莹,王凯,董丛丛,倪辉,杨远帆,黄高凌,蔡慧农,杜希萍..二甲亚砜法破壁条件对法夫酵母虾青素提取效果的影响[J].食品工业科技,2017,38(15):212-217,6.基金项目
国家自然科学基金项目(31501448) （31501448）
福建省教育厅项目(JA15266) （JA15266）
厦门市海洋渔业局(南方海洋中心)项目(14CZP035HJ09). （南方海洋中心）
