食品工业科技2017，Vol.38Issue(15)：248-252,5.DOI:10.13386/j.issn1002-0306.2017.15.046
紫斑牡丹花粉中异鼠李素的定性定量分析及总黄酮的含量测定
Qualitative and quantitative analysis of isorhamnetin and content determination of total flavonoids from pollen of Paeonia rockii
摘要
Abstract
Objective:To establish methods for qualitative and quantitative analysis of isorhamnetin and content determination of total flavonoids from pollen of Paeonia rockii.Methods:The identification of isorhamnetin from pollen of Paeonia rockii was performed by TLC.The content of isorhamnetin was determined by HPLC.Agilent Eclipse plus C18 column(250 mm× 4.6 mm,5 μm)was adopted,the mobile phase was methanol-acetonitrile-0.1％ phosphoric acid(25∶ 13∶62),at the flow rate of 1.0 mL·min-1.The detection wavelength was 360 nm.The content of total flavonoids was deternined by UV-visible spectrophotometry.Results:The TLC method was suitable for the identification of isorhamnetin with good reproducibility.The linear of isorhamnetin was in the range of 8.4～ 168.0 μg· mL 1 (r =0.9999),and the average recovery(n =9) was 100.8％ (RSD =2.36％),the content of isorhamnetin fron pollen of Paeonia rockii was 3.21～8.91 mg· g-1.The linear range of total flavonoids was 0.01～0.06 mg· mL 1(r =0.9992)and the average recovery(n =9)was 101.06％ (RSD =2.56％),the content of flavonoids from pollen of Paeonia rockii was 9.44～ 15.96 mg·g-1.Conclusion:The developed methods are simple,accurate and suitable for the quality control of pollen of Paeonia rockii.关键词
紫斑牡丹花粉/异鼠李素/定性定量分析/总黄酮/薄层色谱法/反相高效液相色谱法/紫外分光光度法Key words
pollen of Paeonia rockii/isorhamnetin/qualitative and quantitative analysis/total flavonoids/TLC/RP-HPLC/UV分类
轻工纺织引用本文复制引用
王新娣,石晓峰,王斌利,范彬,马趣环,刘东彦,沈薇..紫斑牡丹花粉中异鼠李素的定性定量分析及总黄酮的含量测定[J].食品工业科技,2017,38(15):248-252,5.基金项目
兰州市2016年第三批科技计划项目(2016-3-17). （2016-3-17）
