食品工业科技2017，Vol.38Issue(15)：270-274,323,6.DOI:10.13386/j.issn1002-0306.2017.15.050
尼罗红荧光光谱法快速测定解脂耶氏酵母油脂含量的研究
Study on a Nile Red fluorescence spectrometry for rapid lipid determination in Yarrowia lipolytica
摘要
Abstract
In order to establish a rapid lipid determination method in Yarrowia lipolytica,the determination conditions were studied with the Nile Red fluorescence spectrometry.The optimal determination conditions were confirmed by researching the optional emission wavelength in Yarrowia lipolytica,optimal volume fraction,dosage of Nile Red dye solution,dyeing time and efficiency of different penetrating agents,the linearity between lipid content in cell and fluorescence intensity were affirmed.Yarrowia lipolytica had maximum fluorescence value in excitation wavelength 560 nm,emission wavelength 650 nm,20 μL 0.1 mg/mL mass fraction of Nile Red dye solution and 15％ volume fraction of isopropanol were added in per milliliter of Yarrowia lipolytica suspension respectively,dyeing time 5 min in darkness,in the range of cell with OD600of 0～1.3,there was a good linear relationship between lipid content(X) of solution and fluorescence intensity (Y),and the linear formula was Y =6.3651X + 10.097(R2 =0.9902),the sensitivity of this method was 0.0001 g.The Nile Red fluorescence spectrometry could reflect the lipid content accurately in Yarrowia lipolytica and he used as a new method for rapid lipid determination in Yarrowia lipolytica.关键词
解脂耶氏酵母/油脂含量/尼罗红/荧光光谱法Key words
Yarrowia lipolytica/lipid content/Nile Red/fluorescence spectrometry分类
轻工纺织引用本文复制引用
高山,刘相岑,朱晨光,史吉平,张保国..尼罗红荧光光谱法快速测定解脂耶氏酵母油脂含量的研究[J].食品工业科技,2017,38(15):270-274,323,6.基金项目
国家自然科学基金项目(41106125). （41106125）
