食品工业科技2017，Vol.38Issue(16)：118-125,8.DOI:10.13386/j.issn1002-0306.2017.16.023
鱼鳔糖胺聚糖的提取及其吸湿保湿性能评价
Extraction of glycosaminoglycans from swim bladder and evaluation of the capacities of its hygroscopicity and moisture retention
摘要
Abstract
The extraction process of glycosaminoglycan from swim bladder via enzymatic hydrolysis by pancreatin and neutral protease of Bacillus subtilis were optimized by response surface methodology based on single factor experiments,the physicchemical characters and structure properties were analyzed by UV and IR spectroscopy,and the evaluation of the capacities of hygroscopicity and moisture retention were conducted in this investigation.The results showed that the optimum processing conditions of enzyme hydrolysis was solid-liquid ration of 1 ∶ 20 g∶ mL,enzymolysis time of 4 h,enzymolysis temperature of 50 ℃,enzyme dosage of 7％,and the enzmolysis pH of 8.0.Under these optimum conditions,the yield and content of glycosaminoglycan were of 1.19％,19.09％.Moreover,the IR spectrum showed that the extracted glycosaminoglycan had typical characteristic absroption peaks of polysaccride,and comprised a pyranose ring structure having an α glycosidic bond.Finally,the extracted glycosaminoglycan had excellent capacities of hygroscopicity and moisture retention,which were superior to those of the conventional humectants,such as chitosan and sodium alginate.These results clearly established the possibility that swim bladder glycosaminoglycan could be effectively employed as a moisturizer.关键词
鱼鳔/糖胺聚糖/提取工艺/理化及结构特征/吸湿性/保湿性Key words
swim bladder/glycosaminoglycan/extraction process/physic-chemical characters and structure properties/hygroscopicity/moisture retention分类
轻工纺织引用本文复制引用
屈义,周斯仪,冯陶,钟赛意,谌素华,苏伟明,吴小禾..鱼鳔糖胺聚糖的提取及其吸湿保湿性能评价[J].食品工业科技,2017,38(16):118-125,8.基金项目
广东省高等教育“创新强校工程”专项资金(GDOU2016050203) （GDOU2016050203）
2017年广东省海洋和渔业发展专项资金生物医药与功能性制品研发2 （）
中山市淡水产品技术服务协同创新中心项目(2016C1007). （2016C1007）
