食品工业科技2017，Vol.38Issue(16)：126-130,5.DOI:10.13386/j.issn1002-0306.2017.16.024
ARTP诱变选育高产海藻糖菌株及酶促反应条件优化
Breeding of the trehalose high-yielding strain by ARTP and the optimization of enzymatic reaction conditions
摘要
Abstract
A genetic stability trehalose high-yielding strain Arthrobacter sp.SH-52 was obtained from the original strain of Arthrobacter sp.SH,which was mutated by atmospheric and room temperature plasmas (ARTP).The catalysis ability of Arthrobacter sp.SH-52 reached 129.6 U/mL,which was 46.1％ higher than that of the original strain.The enzymatic reaction conditions of SH-52 were optimized,and the results were as follows:reaction temperature 45 ℃,reaction time 30 h,pH6.5,and DE value 11.9 of 20％ starch hydrolysate.Under the optimum reaction conditions,the conversion rate reached 37.6％ by crude enzyme.The results showed that ARTP mutagenesis technology was an effective method for breeding high-activity trehalose-producing bacteria,and the results could be used for industrial production of trehalose.关键词
海藻糖/常压室温等离子体诱变/TreY-TreZ途径/淀粉液化液Key words
trehalose/atmospheric and room temperature plasma(ARTP)/TreY-TreZ pathway/starch hydrolysate分类
轻工纺织引用本文复制引用
田成福,张伟国,徐建中,刘冬冬,史仲平..ARTP诱变选育高产海藻糖菌株及酶促反应条件优化[J].食品工业科技,2017,38(16):126-130,5.基金项目
江苏省自然科学基金-青年基金(BK20150149). （BK20150149）
