食品工业科技2017，Vol.38Issue(16)：142-149,158,9.DOI:10.13386/j.issn1002-0306.2017.16.027
响应面法优化酶解制备核桃多肽工艺
Optimization of enzymatic hydrolysis of walnut peptide by response surface methodology
摘要
Abstract
Walnut peptide was obtained by enzymatic hydrolysis with the walnut powder as raw material.The preparation process of walnut peptide was optimized on foundation of single factor experiment,according to Box-Behnken central composition design principle,the method of response surface analysis was adopted with the peptide mass concentration and degree of hydrolysis as the response value.The effect of pH,temperature,enzyme dosage and time on the peptide mass concentration and degree of hydrolysis were studied in this paper.On the basis of this,different molecular peptides was obtained by enzymatic hydrolysate separated with ultrafiltration system.The antioxidant activity of different molecular peptides was studied.The results showed that the dissolution of protein was sufficient when the walnut-to-water was 1 ∶ 20 g/mL.When the mixed ratio of the alkaline protease and papain was 1∶ 1,the dosage of flavourzyme was 2000 U/g,the hydrolysis effect was better.The optimum preparation conditions were obtained as follows:pH 7.10,temperature 50 ℃,enzyme dosage 8000 U/g and time 3.0 h,the peptide mass concentration was 10.01 mg/mL and the degree of hydrolysis was 11.45％,which was close to the theoretical value.Four kinds of molecular peptides(≤5,5～ 10,10～30 and ≥30 kDa)was obtained by ultrafiltration separation,and both of them had antioxidant activity in vitro.The reducing power(IC50 =5.47 mg/mL),the scavenging activity of the DPPH· (IC50 =1.03 mg/mL),the scavenging activity of the ·OH(IC50 =5.02 mg/mL),the scavenging activity of the O2-· (IC50 =0.68 mg/mL) and the total antioxidant capacity (14.18 U/mL)of the ≤ 5 kDa peptides,was better than the other three kinds of peptides.This study provided certain theoretical guidance for the deep processing and development of walnut products.关键词
核桃/多肽/响应面法/工艺优化/抗氧化活性Key words
walnut/peptide/response surface methodology/process optimization/antioxidant activity分类
轻工纺织引用本文复制引用
陈树俊,李乐,石玥,胡洁,徐晓霞,李佳益,张君梅,王翠连..响应面法优化酶解制备核桃多肽工艺[J].食品工业科技,2017,38(16):142-149,158,9.基金项目
山西省重点研发计划项目(201603D221033-1). （201603D221033-1）
