食品工业科技2017，Vol.38Issue(16)：189-193,198,6.DOI:10.13386/j.issn1002-0306.2017.16.035
金花葵花总黄酮的提取及体外抗氧化活性
Extraction of total flavonoids from Hibiscus manihot L.flower and their antioxidant activity in vitro
摘要
Abstract
This test used solvent extraction of flavonoids from Hibiscus manihot L.flower by using response surface methodology to optimize the extraction conditions,and the antioxidant activity in vitro was evaluated by artificial free radicals in detail.The optimal extraction conditions were as follows:the volume fraction of ethanol 75％,ratio of liquid to solid 30∶ 1,extraction temperature 60 ℃,extraction time 2.5 h.Under this optimum conditions,the actual yield of flavonoids was 126.67 mg/g dry weight.The total flavonoids in Hibiscus manihot L.flower had a good scavenging effect on superoxide anion radical with a 50％ inhibition concentration(IC50)of 207.1 μg/mL and which was better than that of ascorbic acid.The extracts also had a good scavenging effect on DPPH radical and hydroxyl radical with IC50 of 37.5 μg/mL and 754.7 μg/mL respectively,which were a little lower than that of ascorbic acid.The extract also showed a certain reduction ability and it was 83％ times as much as that of ascorbic acid.In general,total flavonoids from Hibiscus manihot L.flower had a high antioxidant activity.关键词
金花葵花/总黄酮/提取/抗氧化活性Key words
Hibiscus manihot L.flower/total flavonoids/extraction/antioxidant activity分类
轻工纺织引用本文复制引用
王菲,孙一焱,解长海,宋力,孙志宇,孙家龙,陆光..金花葵花总黄酮的提取及体外抗氧化活性[J].食品工业科技,2017,38(16):189-193,198,6.基金项目
辽宁省教育厅科学研究一般项目(L2016006). （L2016006）
