食品工业科技2017，Vol.38Issue(16)：229-233,5.DOI:10.13386/j.issn1002-0306.2017.16.043
高产靛蓝色素大肠杆菌工程菌的构建及靛蓝色素稳定性
Construction of gene engineered Escherichia coil strain for high-yield production of indigo and its stability
摘要
Abstract
As the natural food blue pigment sources are comparatively rare,microbial fermentation is an efficient way to produce the natural indigo pigment.In this study,the styrene monooxygenase gene styAB was cloned from Pseudomonas putida B3 and then over-expressed in Escherichia coli,generating the gene engineered strain E-AB for high-yield production of indigo.By fermentation conditions optimization,a maximum yield of 68.9 mg/L of indigo was obtained in E-AB using indole as the substrate,which was 5.4-fold higher than that of wild-type strain B3.The indigo stability was further investigated under different temperature and illumination conditions.High temperature and UV light obviously enhanced indigo degradation,while low temperature and light-proof condition could delay indigo degradation.And it was detected that isatin was the main degradation compound during indigo degradation.These results would provide the theoretical support and technical guidance for high efficient biotransformation preparation and apphcation of the natural indigo pigment.关键词
靛蓝色素/基因工程菌株/苯乙烯单加氧酶/发酵/色素稳定性Key words
indigo/gene engineered strain/styrene monooxygenase/fermentation/pigment stability分类
轻工纺织引用本文复制引用
杜灵燕,张策,车逸心,刘雯娴,王孟菲,尹胜,王成涛..高产靛蓝色素大肠杆菌工程菌的构建及靛蓝色素稳定性[J].食品工业科技,2017,38(16):229-233,5.基金项目
国家自然科学基金项目(31401669,31571801) （31401669,31571801）
北京市自然科学基金项目(5154027) （5154027）
2016年度大学生科学研究与创业行动计划项目(19008001131). （19008001131）
