食品工业科技2017，Vol.38Issue(17)：10-16,23,8.DOI:10.13386/j.issn1002-0306.2017.17.003
丁香乙酸乙酯相抑制LDL中赖氨酸、色氨酸氧化修饰的研究
Inhibition effect of ethyl acetate fraction of clove on Trp and Lys modification during LDL oxidation
摘要
Abstract
Oxidative modification of Tryptophan (Trp) and Lysine (Lys) in apoB-100 of LDL is the important factor resulting in atherosclerosis(AS).In order to investigate the possible inhibition effect of Ethyl acetate fraction of clove(EAFC) on Trp and Lys oxidative modification during LDL oxidation,the concentrations of CuSO4 and LDL were firstly optimized,and then this inhibition effects of EAFC against oxidation were evaluated by using fluorescence as the main index in this paper.The results indicated that the concentrations of CuSO4 and LDL were 1.25,25 μmol/L and 500 μg/mL respectively in subsequent experiments.Fluorescence quenching can be inhibited by 20 μg/mL EAFC,and this inhibition effect was significantly higher than that of the 1 μg/mL butylated hydroxytoluene (positive control,BHT) (p ＜ 0.01).Likewise,3 μg/mL EAFC can inhibit the modification of Lys residues by highly reactive aldehydes.10 μg/mL EAFC can dramatically inhibit covalent binding of 4-HNE with the Lys residues(p ＜0.01).The modification of Lys residues resulting from MDA was inhibited by EAFC(15 μg/mL) which was significant than that of 1 μg/mL BHT.Meanwhile,15 μg/mL EAFC showed a significant inhibition on formation of lipofuscin(p ＜0.01).This study provides references for further analyzing the inhibition way of EAFC and the mechanism that EAFC inhibits the recognition between ox-LDL and scavenger receptors.关键词
丁香/ApoB-100/低密度脂蛋白/色氨酸/赖氨酸Key words
clove/apolipoproteinb-100/low density lipoprotein/tryptophan/lysine分类
轻工纺织引用本文复制引用
万严,杨琼玉,曲文娟,谢丽琴,郝澍,沈勇根,张良慧,江慎华..丁香乙酸乙酯相抑制LDL中赖氨酸、色氨酸氧化修饰的研究[J].食品工业科技,2017,38(17):10-16,23,8.基金项目
国家自然科学基金(31360371,31560308,31301423) （31360371,31560308,31301423）
江西省科技支撑计划(20151BBF60026) （20151BBF60026）
江西省教育厅项目(GJJ161081) （GJJ161081）
江苏省农产品物理加工重点实验室开放课题(JAPP2010-5) （JAPP2010-5）
江西省天然产物与功能食品重点实验室开放基金、九江学院人才启动基金、九江学院教改课题(2015-04). （2015-04）
