食品工业科技2017，Vol.38Issue(17)：17-23,7.DOI:10.13386/j.issn1002-0306.2017.17.004
鱼胶原蛋白酶解工艺及其活性肽抑制ACE酶的研究
Study on enzymatic hydrolysis process of fish collagen and antihypertensive peptides of collagen
摘要
Abstract
Single-factor and orthogonal experiments were employed to optimize the preparation process of collagen polypeptide through the hydrolysis of the fish collagen by pepsin.The angiotensin converting enzyme (ACE) inhibitory activity of marine and freshwater fish collagen was determined by high performance liquid chromatography to characterize its blood pressure-lowering function.It showed that the optimal hydrolysis condition of pepsin was emzyme-to-substrate ratio of 1 ∶ 125,substrate concentration 3％,temperature 37 ℃,initial pH2.5 and reaction time 12 h while DH was 19.17％.The results showed that the supernatant of enzymatic hydrolysis of marine fish collagen and freshwater fish collagen possessed higher ACE inhibitory activity,while the IC50 of their hydrolysates to ACE were 1.34 mg/mL and 1.52 mg/mL.The hydrolysate was separated by Amicon Ultra-4 Centrifugal Filter Devices with the molecular weight lower than 3 ku.The inhibitory activities of the two fractions was determined.The results showed that the IC50 of CP-Ⅰ (≤3 ku) and CP-Ⅲ (≤3 ku) were 0.43 mg/mL and 0.60 mg/mL.While the IC50 of CP-Ⅱ (≥3 ku)and CP-Ⅳ(≥3 ku)were 1.78 mg/mL and 1.69 mg/mL.关键词
血管紧张素转换酶/鱼/胶原蛋白/活性肽Key words
angiotensin converting enzyme/fish/collagen protein/bioactive peptide分类
轻工纺织引用本文复制引用
汪雨亭,黄儒强,王娟..鱼胶原蛋白酶解工艺及其活性肽抑制ACE酶的研究[J].食品工业科技,2017,38(17):17-23,7.基金项目
广东省海洋渔业科技推广专项科技攻关与研发项目(A201301C10). （A201301C10）
