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马铃薯中直链淀粉和支链淀粉含量测定方法的优化

王丽 罗红霞 李淑荣 刘小飞 潘妍 徐毓谦

食品工业科技2017,Vol.38Issue(17):220-223,4.
食品工业科技2017,Vol.38Issue(17):220-223,4.DOI:10.13386/j.issn1002-0306.2017.17.042

马铃薯中直链淀粉和支链淀粉含量测定方法的优化

Optimization for determination of content of amylose and amylopectin in potato

王丽 1罗红霞 1李淑荣 1刘小飞 1潘妍 1徐毓谦1

作者信息

  • 1. 北京农业职业学院,北京102442
  • 折叠

摘要

Abstract

This paper used the potato as material,optimized the pretreatment method,improved measure method of the amylose and amylopectin,completed the potato starch analysis method.Standard material of amylose and amylopectin was used to draw the standard curve.Five samples,including no desugar and no degrease,degrease,desugar,desugar first than degrease,degrease first than desugar,was used to analysis the content of amylose and amylopectin.The result showed that the maximum absorption wavelength of amylose and amylopectin was 609 nm and 546 nm,reference wavelength was 473 nm and 734 nm,respectively.The coefficient of standard equation regression was higher than 0.999.The sample of degrease first than desugar had good solubility,the content of amylopectin and amylose was 70.27% and 12.38%,respectively.So the degrease first than desugar was a good pretreatment method,which can be accuracy analysis the content of amylose and amylopectin at the dual-wavelength.

关键词

马铃薯/直链淀粉/支链淀粉/双波长比色法

Key words

potato/amylose/amylopectin/dual-wavelength colorimetry

分类

轻工纺织

引用本文复制引用

王丽,罗红霞,李淑荣,刘小飞,潘妍,徐毓谦..马铃薯中直链淀粉和支链淀粉含量测定方法的优化[J].食品工业科技,2017,38(17):220-223,4.

基金项目

北京农业职业学院博士基金项目(XY-BS-16-04). (XY-BS-16-04)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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