食品工业科技2017，Vol.38Issue(17)：224-229,6.DOI:10.13386/j.issn1002-0306.2017.17.043
分子印迹固相萃取-超高效液相色谱串联质谱法测定猪肉及猪肝中14种非甾体抗炎药残留量
Determination of 14 nonsteroidal anti-inflammatory drugs by molecularly imprinted polymers solid phase extraction with ultra-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A molecularly imprinted solid phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry method was established for the determination of 14 kinds of nonsteroidal anti-inflammatory drugs in pork and liver.Sample was extracted twice by 10 mmol/L pH3.0 ammonium formate buffer,followed with defat by n-hexane.Supernatant was purified by molecularly imprinted solid phase extraction.Analytes were separated by BEH C18 column under gradient elution,then detected with mass spectrometry under positive and negative electric spray ionization and multiple reactions monitoring mode.The calibration curves of 14 nonsteroidal anti-inflammatory drugs were linear in the range of 0.25～50 μg/L.The limits of detection and quantitation were 0.1～2 μg/kg and 0.25～5 μg/kg,respectively.In three different spike levels,the average recoveries in pork and liver were between 73.2％ ～110.7％,with relative standard deviations of 1.0％～9.7％.The sensitivity,precision and accuracy of this method were in accordance with the demand of veterinary residue determination,it is suitable for the multi residue determination of nonsteroidal anti-inflammatory drugs.关键词
分子印迹固相萃取/液相色谱串联质谱法/非甾体抗炎药/多残留检测Key words
molecularly imprinted solid phase extraction/liquid chromatography-tandem mass spectrometry/nonsteroidal anti-inflammatory drugs/multi residue determination分类
轻工纺织引用本文复制引用
郝杰,姜洁,毛婷,史娜,孙晓冬..分子印迹固相萃取-超高效液相色谱串联质谱法测定猪肉及猪肝中14种非甾体抗炎药残留量[J].食品工业科技,2017,38(17):224-229,6.基金项目
北京市科技计划(Z161100000616007). （Z161100000616007）
