食品工业科技2017，Vol.38Issue(17)：292-295,4.DOI:10.13386/j.issn1002-0306.2017.17.057
毛蕊异黄酮对衰老小鼠学习记忆能力的改善作用及机制研究
Mechanisms and effects of calycosin on learning-memory function in aging mice
摘要
Abstract
Objective:The effects of calycosin on learning-memory behavior in aging mice induced by D-galactose were investigated in this study.Methods:The aging mice model was induced by subcutaneous injection of D-galactose (150 mg·kg-1 ·d-1)were established after 6 weeks.The D-galactose-induced aging mice were divided into model group,oxiracetam group (300 mg· kg-1· d-1),and calycosin high,medium and low dose treated-groups (20,12,7 mg· kg-1· d-1).Mice in the model group and normal control group were given normal saline.After intraperitoneal injection 7 d,the passive learning ability and memory capacity of mouse were investigated by the platform and the Y maze.Then organ index and antioxidant activity were detected,such as liver,spleen,thymus,brain and serum superoxide dismutase (T-SOD),glutathione peroxidase (GSH-Px),malondialdehyde (MDA) and brain acetylcholinesterase (AchE) activity.Results:Compared with the model group,in the Y maze,the number of correct reaction and active avoidance rate of calycosin-treated mice were significantly increased(p ＜ 0.05),and the escape period was significantly shortened and the number of errors in the step-down test was significantly reduced (p ＜ 0.05).Compared with the model group,liver,spleen,thymus and brain organ index of calycosin-treated mice were significantly different (p ＜ 0.05).In the calycosin groups,antioxidant enzyme (T-SOD,GSH-Px) activity was significantly elevated(p ＜ 0.05),MDA content was significantly reduced(p ＜ 0.05),and brain tissue AchE activity was decreased (p ＜ 0.05).Conclusion:Calycosin can improve the learning-memory function of aging mice induced by D-galactose.The mechanism may be to increase the endogenous antioxidant enzyme activity,reduce lipid peroxidation damage,protect organ function such as brain,spleen and thymus and reduce brain tissue AchE activity.关键词
毛蕊异黄酮/D-半乳糖/器官指数/抗氧化/抗衰老Key words
calycosin/D-galactose/organ index/antioxidation/anti-aging分类
轻工纺织引用本文复制引用
于德红,王美,陈金铭,王旭,耿增岩,李颖..毛蕊异黄酮对衰老小鼠学习记忆能力的改善作用及机制研究[J].食品工业科技,2017,38(17):292-295,4.基金项目
河北省教育厅项目(Z2011247). （Z2011247）
