食品工业科技2017，Vol.38Issue(17)：324-328,334,6.DOI:10.13386/j.issn1002-0306.2017.17.063
实时荧光定量PCR技术在肉及肉制品中的应用
Application of quantitative real-time PCR in meat and meat products
摘要
Abstract
Quantitative real-time PCR as a technology for quantitative measurement of DNA has been widely used in the identification and quantitative analysis the species of meat products.Compared with the traditional or physical and chemical identification,quantitative real-time PCR has the advantages of high sensitivity and specificity,simple operation and time saving.The amount of products of the PCR cycle and types of amplified DNA fragments can be determined by the use of TaqMan probe and fluorescence dyes,then types and addition of meat is determined.Therefore,the technology can be applied to the detection of adulteration of meat and meat products safety.The principle of quantitative real-time PCR was introduced briefly,and application of quantitative real-time PCR was mainly reviewed in species identification and bacterial contamination of meat and meat products.Finally,the application of this technology in meat progress was prospected.关键词
TaqMan荧光探针/荧光染料/实时荧光定量PCR/肉制品Key words
TaqMan probe/fluorescence dyes/quantitative real-time PCR/meat products分类
轻工纺织引用本文复制引用
陈益春,牛海力,孔保华..实时荧光定量PCR技术在肉及肉制品中的应用[J].食品工业科技,2017,38(17):324-328,334,6.基金项目
国家自然科学基金(31471599) （31471599）
国家重点研发计划项目(2016YFD0401504). （2016YFD0401504）
