食品工业科技2017，Vol.38Issue(18)：65-68,73,5.DOI:10.13386/j.issn1002-0306.2017.18.013
光谱法研究高良姜素与人血清白蛋白的相互作用
Spectroscopic analysis of interaction between galangin and human serum albumin
摘要
Abstract
Under the imitated physiological condition,the interaction between galangin and human serum albumin (HSA)was studied by fluorescence quenching,synchronous fluorescence,three-dimensional fluorescence and circular dichroism spectra.The results suggested that galangin had a strong ability to quench the HSA fluorescence in a static mode,during which hydrogen bond and Van Edward force played dominant roles.The binding constants (Ka) and site numbers (n) obtained at different temperatures were 1.26 × 106 L/mol,l.17(290.15 K),4.34 × 105 L/mol,1.09(296.15 K),1.23 × 105 L/mol,1.00(303.15 K),9.87 × 104 L/mol,0.99 (310.15 K),respectively.Spectra of synchronous fluorescence,three-dimensional fluorescence and circular dichroism revealed that galangin interacted with tryptophan residues in BSA more strongly than with tyrosine residues,and the vicinity of tryptophan residues was less hydrophobic.However,conformational changes of HAS were slighter.关键词
高良姜素/人血清白蛋白/相互作用/同步荧光光谱/三维荧光光谱/圆二色谱Key words
galangin/human serum albumin/interaction/synchronous fluorescence spectra/three-dimensional fluorescence spectra/circular dichroism spectra分类
轻工纺织引用本文复制引用
薛燕斌,乔华,李波,丁伟,孙体健..光谱法研究高良姜素与人血清白蛋白的相互作用[J].食品工业科技,2017,38(18):65-68,73,5.基金项目
山西省自然科学基金(2015011024). （2015011024）
