食品工业科技2017，Vol.38Issue(18)：79-84,6.DOI:10.13386/j.issn1002-0306.2017.18.016
高产类胡萝卜、素的蛹虫草液体培养基优化及其提取工艺研究
Optimization of the liquid culture medium composition for high carotenoid production by Cordyceps militaris and the investigation on the improvement of carotenoid extraction
摘要
Abstract
The cereal-based medium for liquid fermentation was optimized according to the carotenoids production of three strains of Cordyceps militaris CM-03,CM-04 and CM-11.The media components of this work contained several kinds of grains such as wheat (W),corn (C),bean (B),rice (R),bran (Br) and inorganic salts like MgSO4 · 7 H2O (Mg),KH2 PO4 (KHP) and vitamin B1 (VB1).The results showed that the best culture medium formula for the carotenoids accumulation by Cordyceps militarisCM-03 consisted of W(1.4 g·L-1),C(2.8 g·L-1),B(0.3 g·L-1),Mg(0.1 g·L-1),KHP(0.1 g·L-1)and VB1 (0.05 mg·L-1),with natural pH,with the carotenoids yield of(694.8 ± 15.4) μg/g.For Cordyceps militaris CM-04,its highest carotenoids output was (708.5 ± 25.8) μg/g under the formula consisted of R (1.2 g · L-1),C (0.6 g · L-1),B(0.3 g·L-1),Mg(0.1 g· L 1),KHP(0.1 g· L-1),VB1 (0.05 mg· L-1) with natural pH.The highest carotenoids output of Cordyceps militaris CM-11 was(700.5 ± 19.1) μg/g with natural pH,the formula formed by W(1.7 g·L-1),C(2.6 g·L-1),B(0.3 g·L-1),Mg(0.1 g· L-1),KHP(0.1 g· L-1) and VB1 (0.05 mg·L-1).The extraction process with ethanol was also optimized in this work,and the results showed that the optimal extraction condition was as follows:grinding with quartz sand,ethanol concentration 60％,material to liquid ratio 1:40,water bath temperature 80 ℃C,extraction level 2,and extraction rate of 92.8％.关键词
蛹虫草/类胡萝卜素/液体发酵/培养基/提取Key words
Cordyceps militaris/carotenoids/liquid fermentation/medium/extraction分类
轻工纺织引用本文复制引用
殷林,林俊芳,钱璟,叶志伟,罗诗华,郭丽琼,云帆..高产类胡萝卜、素的蛹虫草液体培养基优化及其提取工艺研究[J].食品工业科技,2017,38(18):79-84,6.基金项目
国家自然科学基金项目(31372116,31572178) （31372116,31572178）
广东省自然科学基金(2016A030313404) （2016A030313404）
广东省科技计划项目(2014B02 0205003 （）
2014B050505018). （）
