食品工业科技2017，Vol.38Issue(18)：85-89,93,6.DOI:10.13386/j.issn1002-0306.2017.18.017
超临界CO2体系中磷脂酶A2催化合成高DHA含量DHA-PC的研究
Catalytic synthesis of DHA-PC with high DHA contents by phospholipase A2 in supercritical CO2 system
摘要
Abstract
With the raw material of euphausia superba powder,the purity of the refining phosphatidylcholine gained by ethyl alcohol extraction and separation and purification reached 90.8％,then the refining phosphatidylcholine was used as raw material and phospholipase A2 was used to catalyze the fatty acid hydrolysis of Sn-2 position of phosphatidylcholine in supercritical CO2 system to make the fish oil DHA to Sn-2 position and form the DHA-PC with high DHA contents.Meanwhile,the effects of different parameters on the reaction were surveyed.The results showed that,the best additive amount of immobilization phospholipase A2 was 15 ％ (occupied the total substratio),the best enzymolysis was 6 h,the best enzymolysis temperature was 55 ℃,the best enzymolysis pressure was 12 MPa,respectively.Under such condition,the DHA binding ratio of euphausia superba phosphatidylcholine could reach 62.3 mol％.And it could be concluded that immobilization phospholipase A2 is highly viable for phosphatidylcholine modification to prepare DHA-PC.关键词
南极磷虾/磷脂酰胆碱/DHA-PC/固定化/PLA2/酯交换/超临界CO2Key words
antarctic krill/phosphatidylcholine/DHA-PC/immobilisation/phospholipase A2/transesterification/supercritical carbon dioxide分类
轻工纺织引用本文复制引用
李珍珍,许飞跃,韩玉谦,林洪,冯晓梅..超临界CO2体系中磷脂酶A2催化合成高DHA含量DHA-PC的研究[J].食品工业科技,2017,38(18):85-89,93,6.基金项目
国家自然科学基金(31071541) （31071541）
长江学者与创新研究团队发展计划(TRT1188). （TRT1188）
