食品工业科技2017，Vol.38Issue(18)：90-93,4.DOI:10.13386/j.issn1002-0306.2017.18.018
大豆主要过敏原β-伴大豆球蛋白β亚基的抗原表位分析及分段克隆
Analysis of antigenic epitopes and cloning of soybean major allergen β-conglycinin beta-subunit
摘要
Abstract
The model of tertiary ofβ-conglycinin beta-subunit was constructed by the bioinformation tool SWISS-MODEL,and the conformational epitopes were predicted by DiscoTope 2.0 server.The overlapping fragments of β-conglycinin beta-subunit genes were amplified by PCR and inserted into pMD-18T vector,then preserved into competent cell of Escherichia coli JM109.The recombinant plasmids were evaluated by blue-white selection,PCR and double enzyme digestion,and DNA sequence analysis showed that the cloned sequence was the very fragment designed.The successful cloning of the genes can provide a reference for the study of the expresstion of β-conglycinin beta-subunit and screening of antigenic epitopes.关键词
β-伴大豆球蛋白/β亚基/生物信息学/抗原位点/克隆Key words
β-conglycinin/beta-subunit/bioinformation/antigenic epitopes/clone分类
轻工纺织引用本文复制引用
皮江一,席俊,贺梦雪,李爽..大豆主要过敏原β-伴大豆球蛋白β亚基的抗原表位分析及分段克隆[J].食品工业科技,2017,38(18):90-93,4.基金项目
国家自然科学基金项目(31671778 （）
31301409) （）
河南省高等学校重点科研项目(16A550001). （16A550001）
