食品工业科技2017，Vol.38Issue(18)：105-110,116,7.DOI:10.13386/j.issn1002-0306.2017.18.021
抑菌活性乳酸菌的筛选及其抑菌物质的提取条件优化
Screening of lactic acid bacteria with antibacterial activity and optimization of extraction conditions of antimicrobial substances
摘要
Abstract
Screening on lactic acid bacteria with antibacterial activity,isolated from fermented pickle in eastern Inner Mongolia,was tested for Escherichia coli and Staphylococcus aureus as thc indicator,and its antibacterial spectrum was tested.The optimum separation conditions of antibacterial substances were determined by single factor and L9 (34) orthogonal test,and detected antibacterial effect in commercial cattle feed.The result showed that the strain S1-4 had strong and stable antibacterial activity in 14 strains of lactic acid bacteria,and the diameter of inhibition zone of strain S1-4 to Escherichia coli and Staphylococcus aureus was (19.69 ± 0.71) mm and (20.59 ± 0.26) mm,respectively,and showed broad antimicrobial spectrum.The optimal extraction conditions for the antibacterial substances from strain S1-4 were as follows:the precipitation of pH was 5,the concentration of ethanol was 95％,the settling time was 8 h,and the ratio of material to liquid was 1∶4 (v∶ v).In these conditions,the diameter of the inhibition zone was 39.53 mm,and increased by 23.18％.The fermentation broth of strain S1-4 was added to the cattle feed,which could inhibit the growth of bacteria and mould in feed,and with the increase of the dosage,the inhibitory effect increased obviously.关键词
乳酸茵/抑菌活性/抑菌物质/提取/饲料Key words
lactic acid bacteria/antibacterial activity/antimicrobial substances/extract/feed分类
轻工纺织引用本文复制引用
双全,折米娜,薄礼娟..抑菌活性乳酸菌的筛选及其抑菌物质的提取条件优化[J].食品工业科技,2017,38(18):105-110,116,7.基金项目
国家自然科学基金(31460443) （31460443）
内蒙古自治区自然科学基金项目(2016MS0338). （2016MS0338）
