食品工业科技2017，Vol.38Issue(18)：111-116,6.DOI:10.13386/j.issn1002-0306.2017.18.022
海带内生真菌的分离鉴定及其抑制乙酰胆碱酯酶活性的发酵工艺优化
Isolation, identification and fermentation process optimization of acetylcholinesterase inhibitory activity of endophytic fungus from Laminaria japonica
摘要
Abstract
In this study,Laminaria japonica from Qingdao sea area was used to isolate and purify endophytic fungus.A total of seven strains of endophytic fungi were isolated.Using modified Ellman method to determine the inhibitory rate of AChE and active strains were identified.Strain ML-X showed the highest AChE inhibitory activity and was identified,by physiologicalbiochemical characteristics and molecular biological techniques of 18S rDNA sequencing.Based on single factor and orthogonal test,the culture conditions of liquid fermaentation of the endophytic fungi were screened out.The results showed that the strain ML-X was confirmed as Geotrichum candidum,and the optimal fermentation conditions were as follows:PS medium was the suitable medium for the strain at pH6.0,incubated with 5％ inoculum and 150 mL/500 mL culture volume at 31 ℃ for 8 days.Under these conditions,the strain ML-X exhibited a higher AChE inhibitory activity,the inhibition ratio of AchE was 28.91％ ±0.25％ when the concentration of crude extract from fermentation broth was 5 mg/mL.关键词
内生真菌/鉴定/乙酰胆碱酯酶/抑制活性/条件优化Key words
endophytic fungi/strain identification/acetylcholinesterase/inhibitory activity/conditions optimization分类
轻工纺织引用本文复制引用
田淑娟,阙斐,侯竹美,李飞,王凤舞..海带内生真菌的分离鉴定及其抑制乙酰胆碱酯酶活性的发酵工艺优化[J].食品工业科技,2017,38(18):111-116,6.基金项目
山东省自然科学基金面上项目(ZR2015BM016). （ZR2015BM016）
